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protein microarray analysis proteome profiler tm array  (R&D Systems)


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    R&D Systems protein microarray analysis proteome profiler tm array
    Figure 10 The results of protein <t>microarray</t> analysis. This assay was conducted by using protein microarray analysis. The results are shown in (A) 94T778 cells and (B) SW872 cells. The downregulation of phospho-Akt (Ser473), phospho-mTOR (Ser2448) and phospho-PRAS40 (Thr246) in Lenti-shRRM2 cells of 94T778 and the downregulation of phospho-mTOR (Ser2448) and phospho-PRAS40 (Thr246) in Lenti-shRRM2 cells of SW872 were detected (marked by red frame).
    Protein Microarray Analysis Proteome Profiler Tm Array, supplied by R&D Systems, used in various techniques. Bioz Stars score: 98/100, based on 944 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/phospho-protein+microarray+analysis/Proteome+Profiler+Human+Phospho-Kinase+Array+Kit/10__2147_slash_ott__s246613-91-0-11
    Average 98 stars, based on 944 article reviews
    protein microarray analysis proteome profiler tm array - by Bioz Stars, 2026-10
    98/100 stars

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    1) Product Images from "

    Downregulation of RRM2 Attenuates Retroperitoneal Liposarcoma Progression via the Akt/mTOR/4EBP1 Pathway: Clinical, Biological, and Therapeutic Significance

    "

    Article Title:

    Downregulation of RRM2 Attenuates Retroperitoneal Liposarcoma Progression via the Akt/mTOR/4EBP1 Pathway: Clinical, Biological, and Therapeutic Significance

    Journal: OncoTargets and Therapy

    doi: 10.2147/ott.s246613

    Figure 10 The results of protein microarray analysis. This assay was conducted by using protein microarray analysis. The results are shown in (A) 94T778 cells and (B) SW872 cells. The downregulation of phospho-Akt (Ser473), phospho-mTOR (Ser2448) and phospho-PRAS40 (Thr246) in Lenti-shRRM2 cells of 94T778 and the downregulation of phospho-mTOR (Ser2448) and phospho-PRAS40 (Thr246) in Lenti-shRRM2 cells of SW872 were detected (marked by red frame).
    Figure Legend Snippet: Figure 10 The results of protein microarray analysis. This assay was conducted by using protein microarray analysis. The results are shown in (A) 94T778 cells and (B) SW872 cells. The downregulation of phospho-Akt (Ser473), phospho-mTOR (Ser2448) and phospho-PRAS40 (Thr246) in Lenti-shRRM2 cells of 94T778 and the downregulation of phospho-mTOR (Ser2448) and phospho-PRAS40 (Thr246) in Lenti-shRRM2 cells of SW872 were detected (marked by red frame).

    Techniques Used: Microarray

    Related Articles

    Phospho-proteomics:

    Article Title: Dual inhibition of HDAC and EGFR signaling with CUDC-101 induces potent suppression of tumor growth and metastasis in anaplastic thyroid cancer.
    Article Snippet: Following antibodies were used: anti-p21 (1:500), anti-vimentin (1:5,000), anti-E-cadherin (1:250), antisurvivin (1:2,000), anti-phospho-Erk1/2 (1:1,000), and anti-Erk1/2 (1:1,000)were from Cell Signaling Technology (Boston, MA); anti-N-cadherin (1:4,000) was from EMD Millipore (Billerica, MA); anti-β-catenin (1:250) and antiXIAP (1:1,000) were from R&D Systems (Minneapolis, MN); and anti-β-actin (1:3,000) was from Santa Cruz Biotechnology (Dallas, TX). .. Phospho-kinase and apoptosis protein arrays Human phospho-kinase (Catalog # ARY003B) array (detecting site-specific phosphorylation of 43 kinases and 2 related total proteins, and apoptosis array (Catalog # ARY009 detecting the expression level of 35 apoptosisrelated proteins) were purchased from R&D Systems (Minneapolis, MN). ..

    Expressing:

    Article Title: Dual inhibition of HDAC and EGFR signaling with CUDC-101 induces potent suppression of tumor growth and metastasis in anaplastic thyroid cancer.
    Article Snippet: Following antibodies were used: anti-p21 (1:500), anti-vimentin (1:5,000), anti-E-cadherin (1:250), antisurvivin (1:2,000), anti-phospho-Erk1/2 (1:1,000), and anti-Erk1/2 (1:1,000)were from Cell Signaling Technology (Boston, MA); anti-N-cadherin (1:4,000) was from EMD Millipore (Billerica, MA); anti-β-catenin (1:250) and antiXIAP (1:1,000) were from R&D Systems (Minneapolis, MN); and anti-β-actin (1:3,000) was from Santa Cruz Biotechnology (Dallas, TX). .. Phospho-kinase and apoptosis protein arrays Human phospho-kinase (Catalog # ARY003B) array (detecting site-specific phosphorylation of 43 kinases and 2 related total proteins, and apoptosis array (Catalog # ARY009 detecting the expression level of 35 apoptosisrelated proteins) were purchased from R&D Systems (Minneapolis, MN). ..

    other:

    Article Title: Synbiotic combination of prebiotic grape pomace extract and probiotic Lactobacillus sp. reduced important intestinal inflammatory markers and in-depth signalling mediators in lipopolysaccharide-treated Caco-2 cells
    Article Snippet: Protein array analysis (signalling markers) Human Phospho-MAPK Array kit (R&D Systems) was used to detect the relative levels of phosphorylation of MAPK, ERK1/2, JNK and p38 isoforms, according to the manufacturer’s protocol.

    Article Title: Tobacco Smoke Condensate Induces Morphologic Changes in Human Papillomavirus-Positive Cervical Epithelial Cells Consistent with Epithelial to Mesenchymal Transition (EMT) with Activation of Receptor Tyrosine Kinases and Regulation of TGFB
    Article Snippet: Protein extractions obtained from vehicle- and CSC-treated cells at 72 h were used to conduct RTK phospho-protein array analysis (R&D Systems, CAT# ARY001B, Minneapolis, MN, USA), with each array containing 49 specific antibodies for human RTKs.

    Microarray:

    Article Title: <p>Downregulation of RRM2 Attenuates Retroperitoneal Liposarcoma Progression via the Akt/mTOR/4EBP1 Pathway: Clinical, Biological, and Therapeutic Significance</p>
    Article Snippet: .. Protein Microarray Analysis Proteome Profiler TM Array—Human Phospho-Kinase Array Kit (ARY003B, R&D Systems, Minneapolis, MN) was used to conduct protein microarray analysis in accordance with the manufacturer’s instructions. ..



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    Figure 10 The results of protein <t>microarray</t> analysis. This assay was conducted by using protein microarray analysis. The results are shown in (A) 94T778 cells and (B) SW872 cells. The downregulation of phospho-Akt (Ser473), phospho-mTOR (Ser2448) and phospho-PRAS40 (Thr246) in Lenti-shRRM2 cells of 94T778 and the downregulation of phospho-mTOR (Ser2448) and phospho-PRAS40 (Thr246) in Lenti-shRRM2 cells of SW872 were detected (marked by red frame).
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    Image Search Results


    EV-mediated transfer of proteins via Kinexus phospho-protein microarray analysis in DU145 cells co-cultured with PrEC EVs

    Journal: Molecular Cancer

    Article Title: Reversal of chemosensitivity and induction of cell malignancy of a non-malignant prostate cancer cell line upon extracellular vesicle exposure

    doi: 10.1186/1476-4598-12-118

    Figure Lengend Snippet: EV-mediated transfer of proteins via Kinexus phospho-protein microarray analysis in DU145 cells co-cultured with PrEC EVs

    Article Snippet: To determine the proteins that are involved in or might be responsible for “phenotypic switching”, we utilized Kinexus phospho-protein microarray analysis (Vancouver, BC) in the DU145 cells co-cultured with PrEC EVs.

    Techniques: Microarray

    Figure 10 The results of protein microarray analysis. This assay was conducted by using protein microarray analysis. The results are shown in (A) 94T778 cells and (B) SW872 cells. The downregulation of phospho-Akt (Ser473), phospho-mTOR (Ser2448) and phospho-PRAS40 (Thr246) in Lenti-shRRM2 cells of 94T778 and the downregulation of phospho-mTOR (Ser2448) and phospho-PRAS40 (Thr246) in Lenti-shRRM2 cells of SW872 were detected (marked by red frame).

    Journal: OncoTargets and Therapy

    Article Title:

    Downregulation of RRM2 Attenuates Retroperitoneal Liposarcoma Progression via the Akt/mTOR/4EBP1 Pathway: Clinical, Biological, and Therapeutic Significance

    doi: 10.2147/ott.s246613

    Figure Lengend Snippet: Figure 10 The results of protein microarray analysis. This assay was conducted by using protein microarray analysis. The results are shown in (A) 94T778 cells and (B) SW872 cells. The downregulation of phospho-Akt (Ser473), phospho-mTOR (Ser2448) and phospho-PRAS40 (Thr246) in Lenti-shRRM2 cells of 94T778 and the downregulation of phospho-mTOR (Ser2448) and phospho-PRAS40 (Thr246) in Lenti-shRRM2 cells of SW872 were detected (marked by red frame).

    Article Snippet: Protein Microarray Analysis Proteome Profiler TM Array—Human Phospho-Kinase Array Kit (ARY003B, R&D Systems, Minneapolis, MN) was used to conduct protein microarray analysis in accordance with the manufacturer’s instructions.

    Techniques: Microarray

    Effect of Lem4 on tyrosine phosphorylation of signal proteins in HEK293T cell line, as detected by tyrosine phosphorylation ProArray (Full Moon BioSystems). Phosphorylation levels were increased by at least 30% at 30 sites and decreased by at least 30% at four sites in the cells overexpressing Lem4-GFP. Red dashed line indicates the level of phosphorylation in the control GFP-expressing cell line.

    Journal: The Journal of Biological Chemistry

    Article Title: Legionella pneumophila effector Lem4 is a membrane-associated protein tyrosine phosphatase

    doi: 10.1074/jbc.RA118.003845

    Figure Lengend Snippet: Effect of Lem4 on tyrosine phosphorylation of signal proteins in HEK293T cell line, as detected by tyrosine phosphorylation ProArray (Full Moon BioSystems). Phosphorylation levels were increased by at least 30% at 30 sites and decreased by at least 30% at four sites in the cells overexpressing Lem4-GFP. Red dashed line indicates the level of phosphorylation in the control GFP-expressing cell line.

    Article Snippet: Phospho-specific protein microarray analysis Tyrosine phosphorylation ProArray was purchased from Full Moon Biosystems (Sunnyvale, CA).

    Techniques: Phospho-proteomics, Control, Expressing